3D real-time dynamic microscopy of cells

Digital Holographic Microscopy (DHM) generates, in real-time, high resolution 3D digital images of a sample using the principle of holography. DHM T1000 series with their transmission configuration are ideal instruments for transparent or semi-transparent samples such as micro-lenses and biological materials.

Instrument Introduction

The cell 3D real-time dynamic microscope "DHM (Digital Holographic Microscopy)" of Swiss Lyncee tec is an epoch-making high-tech product. For the first time in scientific history, the digital holographic microscope can directly observe the resolution of the nanometer scale and obtain the three-dimensional morphology of the sample instantly, and it is a non-contact and non-destructive measurement.

The T1000 series is mainly used in the field of life sciences, micro-optics and microfluidics. In the field of life sciences, DHM can directly provide accurate ecological measurements. No additional contrast agent is required and very low illumination power is used. It is very sensitive to small morphological changes and changes in intracellular components. Therefore, DHMT1001 is an ideal tool for efficient screening and real-time monitoring. The interference pattern generated by the light irradiated on the sample and the reference light is saved as digital data using a CCD camera, and three-dimensional data is calculated from it. The dedicated software "Koala Software" is used to calculate the three-dimensional data.

Key Features:

• 3D high-resolution real-time imaging
• Stable and easy to use

Technical specifications

system

• Image type: Phase contrast image with intensity quantification
• Light source: single wavelength laser light source
• Sample stage: Manual adjustment of 3 axes X, Y, Z, adjustable 25mm

Optional: Wider adjustment range

Optional: Software control management of 2&3 axes

• Camera: 1392 x 1040 pixel, 8 bits
• Available objectives: 1.25x, 2.5x, 5x, 10x, 20x, 50x, 63x, 100x

Optional: long working distance objective lens, oil immersion objective lens

• Objective assembly: Single lens wedge assembly, 2 objective slides or 4 objective revolving disks.
• Computer: Latest Pentium series computer Windows XP Professional operating system Optimized DHM, 19-inch display with 1280 x 1024 pixels
• Software: Lyncee Tec's Koala software, based on C++ and .NET technologies. For Windows XP. 3D surface topography step height and roughness measurement.
• Optional working modes: vertical scanning and stroboscopic observation mode

performance

• Vertical resolution: Temporal: 0.2° (0.2nm in air) Spatial: 0.6° (0.6nm in air)
• Vertical digital focus range: 50 times depth of field (depending on objective lens)
• Vertical measurement range: Maximum viewing depth for smooth samples, up to 340nm (vertical scanning mode is required for larger depths)
• Lateral resolution (**): Depends on the objective: 300 nm for oil immersion objective (1.4 NA)
• Observation area: Depends on the objective lens, up to 4.40 mm
• Spatial sampling: 1024 x 1024 pixels (hologram)
• Image acquisition speed: Real-time image: 15fps (512 x 512 pixels), 4 fps (1024 x 1024 pixels)
• Reconstruction delay: 15 fps (1024 x 1024) (optional up to 10000 fps)
• Sample illumination: minimum 1μW/cm2
• Maximum sample size: 50mm x 150mm
• Working distance: depends on the objective lens: 0.3～20mm
• Capture time: Single image capture is less than 1μs

Power Requirements

• Input Voltage: 85-260VAC - 50/60Hz
• Power: less than 120W

Size and weight

• Microscope: 400 x 400 x500mm 34.5 kg